Many tissues such as muscle, skin, liver, and peripheral nerve, have remarkable ability to repair and regrow after injury.
However, the CNS (brain and spinal cord) is limited in its ability to repair or regrowth causing permanent brain damage or paralyses.
Most recently an inhibitory myelin protein, Nogo (Neurite outgrowth), has been cloned and characterized.
It may help block the regeneration of the CNS.
Nogo is the 4th member of reticulon (Rtn) family.
There are three alternative isoforms of Nogo, designated Nogo-A (full length human protein 1192 aa; calculated mol wt 135kD; rat 1163 aa), an intermediate form Nogo-B (373 aa; ~37 K, lacks 186-1004 aa within the extracellular domain), and a shorter form Nogo-C (199 aa; ~25 K, similar to rat vp20 and foocen-s; lacks 186-1004 aa but which has a smaller, alternative N-terminal domain).
Nogo-A has a putative extracellular domain of 1024aa, 2-3 TM domains, and a short C-terminus of 43aa.
Nogo-A is localized to the CNS-myelin, and is highly expressed in oligodendorcytes but not by Schwann cells.
Nogo-B and Nogo-C have been found in several non-neuronal tissues (skeletal muscle, kidney, skin, lung, and spleen), and it may be the 35kD protein recognized by IN-1 antibody.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000.
The calculated mol wt of Nogo-A is ~135kD.
However, a MW of ~180kD has been reported for full length Nogo-A (1)
ELISA: 1:10K-1:100K using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: N2116-21
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes Neurite Outgrowth Inhibitor Protein A (NogoA). NogoA11 epitope is not present in alternatively spliced shorter forms of Nogo-B and Nogo-C. Species Seqeunce Homology: mouse: 94%; human: 83%. No significant sequence homology is detected with Rtn family members.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：An 18aa peptide in the mid-region of rat Nogo-A (KLH coupled).
