84-1047-01　NHE6 (Na, H Exchanger 6) 100ug　N2020-55

特徴

• Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues.
• The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+.
• They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation.
• These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders.
• At least 7 NHE isoforms (NHE1-7) have been cloned so far.
• They are all similar in their primary structure and predicted to have 10-12 transmembrane domains.
• The C-terminal domain of NHEs are predicted to be intracellular
• A mammalian homolog of yeast NHA2 has been identified and classified as NHE6 (KIAA0267).
• NHE6 is 669 aa (mol wt ~75 kD), and predicted to contain 12 TM domains within the cytoplasmic N-terminus, the C-terminus may reside in the matrix.
• NHE6 is ubiquitously expressed but it is most abundant in mitochondrial-rich tissues such as brain, skeletal muscle, and heart.
• Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100,000 using 50-100ng control peptide/well.
• Western Blot: 1-10ug/ml using Chemiluminescence.
• NHE6 has a calculated mol wt of ~75kD
• Optimal dilutions to be determined by the researcher.
• Recommended Control Peptide:N2020-60: NHE6, Human (Na, H Exchanger 6, KIAA0267)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.