Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues.
The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+.
They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation.
These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders.
At least 7 NHE isoforms (NHE1-7) have been cloned so far.
They are all similar in their primary structure and predicted to have 10-12 transmembrane domains.
The C-terminal domain of NHEs are predicted to be intracellular.
A mammalian homolog of yeast NHA2 has been identified and classified as NHE6 (KIAA0267).
NHE6 is 669 aa (mol wt ~75 kD), and predicted to contain 12 TM domains within the cytoplasmic N-terminus, the C-terminus may reside in the matrix.
NHE6 is ubiquitously expressed but it is most abundant in mitochondrial-rich tissues such as brain, skeletal muscle, and heart.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:100,000 using 5-10ng control peptide/well.
Control peptide is N2020-60
Western Blot: 1:1000-1:5000.
NHE6 has a calculated mol wt of ~100kD.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes human NHE6. No significant homology exists with other NHE isoforms (NHE1-5). Species sequence homology: Not established.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 18aa sequence near C-terminus of human NHE6 (KLH coupled).
