84-1046-98　NHE5 (Na, H Exchanger 5) 100ul　N2019-09

特徴

• Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues.
• The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+.
• They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation.
• These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, including hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders.
• Five NHE isoforms (NHE1-5) have been cloned so far.
• They are all similar in their primary structure and predicted to have 10-12 transmembrane domains.
• The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.
• NHE5 (chromosome 16q22.1) is expressed in human brain, testis, spleen and skeletal muscle.
• It is involved in pH regulation to eliminate acids generated by active metabolism.
• It is a major proton extruding system driven by the inward sodium ion chemical gradient (1).
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:50,000.
• Control peptide (N2019-13) can be used to coat ELISA plates at 1ug/ml
• Western Blot: 1:1000-1:5000.
• A band of 95-110kD has been detected in brush border membrane.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.