84-1046-95　NHE4 (Na, H Exchanger 4) 100ul　N2018-07

特徴

• Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues.
• The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+.
• They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation.
• These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders.
• At least 7 NHE isoforms (NHE1-7) have been cloned so far.
• They are all similar in their primary structure and predicted to have 10-12 transmembrane domains.
• The C-terminal domain of NHEs are predicted to be intracellular.
• NHE4 (rat, 717aa; rat chromosome 9 and human chromosome 2) expression is most abundant in rat stomach followed by colon and intestine, and in low levels in rat kidney, uterus, and brain (1).
• Unlike other NHEs, NHE4 has no amiloride-sensitive Na+ uptake under basal or acid-loaded isomotic conditions.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100,000 using 5-10ng control peptide/well.
• Control peptide is N2018-09
• Western Blot: 1:1000-1:5000
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.