Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues.
The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+.
They play a major role in regulation of intracellular pH (pHi) in addition to transcellular absorption of Na+, cell volume regulation and possibly in cell proliferation.
These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, including hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders.
At least 7 NHE isoforms (NHE1-7) have been cloned so far.
They are all similar in their primary structure and predicted to have 10-12 transmembrane domains.
The C-terminal domain of NHEs are predicted to be intracellular.
NHE3 (rat, 831aa; human chromosome 5p15.3) is involved in trans-epithelial sodium absorption.
The NHE3 mRNA is found in kidney cortex, medulla, jejunum, ileum, colon and stomach.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL; A band of ~90kD has been detected in brush border membrane (2).
ELISA: 1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide: N2017-31
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes rat NHE3. No significant sequence homology exist with other NHE isoforms (NHE1, 2, 4 and 5).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide consisting of 22aa within the cytoplasmic, C-terminal domain of rat NHE3. Species Sequence Homology: Mouse: 90%; human: 76%.
