84-1046-91　NHE3 (Na, H Exchanger 3) 100ul　N2017-25

特徴

• Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues.
• The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+.
• They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation.
• These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders.
• Seven NHE isoforms (NHE1-7) have been cloned so far.
• They are all similar in their primary structure and predicted to have 10-12 transmembrane domains.
• The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.
• NHE3 (rat, 831aa; human chromosome 5 p15.3) is involved in trans-epithelial Na+ absorption.
• The NHE3 mRNA is found in kidney cortex, medulla, jejunum, ileum, colon and stomach (1).
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL.
• A band of 95-110kD has been detected in brush border membrane (2)
• ELISA: 1:100,000 Control peptide can be used to coat ELISA plates at 1ug/ml.
• Optimal dilutions to be determined by the researcher.
• Control Peptide: N2017-29
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.