84-1046-89　NHE2 (Na, H Exchanger 2) 100ul　N2016-05

特徴

• Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues.
• The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+.
• They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation.
• These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders.
• Five NHE isoforms (NHE1-5) have been cloned so far.
• They are all similar in their primary structure and predicted to have 10-12 transmembrane domains.
• The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.
• NHE2 (rat 813 aa; human chromosome 2 and rat chromosome 9) has been implicated in volume regulation in renal inner medullary collecting duct cells.
• Its mRNA is found in kidney medulla, cortex, colon, jejunum, ileum, human jejunum, ileum, duodenum, stomach and adrenal glands (1).
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100,000 using 5-10ng control peptide/well.
• Control peptide is N2016-06
• Western Blot: 1:1000-1:5000.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.