Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues.
The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+.
They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation.
These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders.
At least 7 NHE isoforms (NHE1-7) have been cloned so far.
They are all similar in their primary structure and predicted to have 10-12 transmembrane domains.
The C-terminal domain of NHEs are predicted to be intracellular.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:N2015-05
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS and 0.05% sodium azide, before the addition of glycerol to 40%
Specificity：Recognizes the 22aa sequence of NHE1 in human, mouse, rat, bovine, pig and rabbit. 90% homology with hamster.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rb Rt
Immunogen：A 22aa peptide within the cytoplasmic, c-terminal domain of the rat NHE1, coupled to KLH.
