Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues.
The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+.
They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation.
These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders.
At least 7 NHE isoforms (NHE1-7) have been cloned so far.
They are all similar in their primary structure and predicted to have 10-12 transmembrane domains.
The C-terminal domain of NHEs are predicted to be intracellular.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL.
A band of 95-110kD has been detected in brush border membrane
ELISA: 1:100,000; using 50-100ng control peptide per well
Optimal dilutions to be determined by the researcher.
Control Peptide:N2015-09: NHE1, Rat (Na, H Exchanger 1) (Control Peptide)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：chicken
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes rat NHE1. No significant sequence homology exist with other NHE isoforms (NHE2-5).
Isotype：IgY
Calc Applications Abbrev：E
Calc Crossreactivity：Bo Rt
Immunogen：Synthetic peptide corresponding to 22a within the cytoplasmic, c-terminal domain of the rat NHE1, conjugated to KLH. Species Sequence Homology: human, mouse, bovine, porcine and rabbit; 100%; hamster; 90%.
