Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL.
A band of 95-110kD has been detected in brush border membrane
ELISA: 1:10,000-50,000.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:N0035-24E NaPi-2A, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size：100ul
Host：chicken
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human NaPi-2A (Npt IIa).
Isotype：IgY
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A 21 AA synthetic peptide within the putative, cytoplasmic, C-terminus of Human NPT2a (KLH). Species sequence homology: rat, mouse, rabbit-100%, sheep-86%, bovine-81% and opossum-71% NPT2a. No significant sequence homology is observed with NPT2b or other Pi-transporters (Type-I, III, or IV) transporters.
