Inorganic phosphate (Pi) levels are hormonally regulated that affects the physiological activity of bone, kidney, and small intestine.
Majority of the Pi is absorbed in the small intestine and reabsorbed in the proximal tubules in the kidney.
At least 4 groups of structurally and funcationally related proteins are in involved in Pi transport: Type I-related NaPi transporters designated NPT1, Npt1, and NaPi-1 respectively in humans, mouse, and rabbit are expressed in the kidney and liver.
Its expression and activity are not regulated by Pi deprivation or parathyroid hormone (PTH) and its role in Pi-homeostasis is not clear.
Type IIa-related cotransporters, designated NaPi-2 in rat, NaPi-3 or NPT2 in humans, NaPi-4 in opposum, NaPi-5 in flounder vessel, NaPi-6 or Npt-2 in mouse, and NaPi-7 in rabbit, is the primary target for Pi regulation by dietary, hormonal, and tubular Pi reabsorption.
Deletion of Npt2 gene produces severe Pi wasting.
Type II transporters are expressed in kidney, brain, lung, bone and small intestine.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot:1-10ug/ml (ECL)
ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:N0035-24J: NaPi-2A, Human, Control Peptide (Npt IIa)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes human NaPi-2A (Npt IIa). No significant sequence homology is observed with Type NPT2b other Pi-transporters (Type-I, III, or IV) transporters.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A 21 AA synthetic peptide within the putative, cytoplasmic, C-terminus of Human NPT2a was synthesized, conjugated to KLH. Species sequence homology: conserved in rat, mouse, rabbit 100%, sheep 86%, bovine 81% and in opossum 71%.
