Inorganic phosphate (Pi) levels are hormonally regulated that affects the physiological activity of bone, kidney, and small intestine.
Majority of the Pi is absorbed in the small intestine and reabsorbed in the proximal tubules in the kidney.
At least 4 groups of structurally and funcationally related proteins are in involved in Pi transport.
Type IIa-related cotransporters, designated NaPi-2 in rat, NaPi-3 or NPT2 in humans, NaPi-4 in opposum, NaPi-5 in flounder vessel, NaPi-6 or Npt-2 in mouse, and NaPi-7 in rabbit, is the primary target for Pi regulation by dietary, hormonal, and tubular Pi reabsorption.
Deletion of Npt2 gene produces severe Pi wasting.
Type II transporters are expressed in kidney, brain, lung, bone and small intestine.
Type IIb, designated as NaPi-IIB or NaPi-2b in rat/mouse, and NaPi-3b in human, is closely related isoform of the NaPi-2 family.
It is expressed in small intestine and lung.
Human homolog are now desganted as PiT1 (also known as GALV receptors or GLVR1) and PiT2 (A-MuLV receptors or GLVR2 or Ram1).
Pit1 and Pit2 are ~62% related and predicted to contain 10 TM domains that is in contrast with Type 1/2 transporters (6-8 TM).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 Using chemiluminescence.
Apparent molecular weight is 80-90kD and under reducing conditions 2 bands of 40-50kD are observed.
May also form multimeric structures(170 and 200 kD)
ELISA: 1:10,000-50,000 N0035-25B Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes human NaPi-2A. Therefore, antibodies are expected to detect both NPT2 Type a and Type b. No significant sequence homology of is observed with other Pi-transporters (Type-I, III, or IV) transporters.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to aa206-225 within the 2nd extracellular loop (between TM3-4) of human NPT2 (KLH). Species sequence homology: rat, mouse, sheep-100%, opposum-95%, chicken, zebra fish, frog-90%. Sequence homology: mouse, human and other species NPT2b ~90% .
