Inorganic phosphate (Pi) levels are hormonally regulated, affecting the physiological activity of bone, kidney, and small intestine.
Majority of the Pi is absorbed in the small intestine and reabsorbed in the proximal tubules in the kidney.
At least 4 groups of structurally and functionally related proteins are in involved in Pi transport: Type I-related NaPi transporters designated NPT1, Npt1, and NaPi-1 respectively in humans, mouse, and rabbit are expressed in the kidney and liver.
Its expression and activity are not regulated by Pi deprivation or parathyroid hormone (PTH) and its role in Pi-homeostasis is not clear.
Type IIa-related cotransporters, designated NaPi-2 in rat, NaPi-3 or NPT2 in humans, NaPi-4 in opossum, NaPi-5 in flounder vessel, NaPi-6 or Npt-2 in mouse, and NaPi-7 in rabbit, is the primary target for Pi regulation by dietary, hormonal, and tubular Pi reabsorption.
Deletion of Npt2 gene produces severe Pi wasting.
Type II transporters are expressed in kidney, brain, lung, bone and small intestine.
Type IIb, designated as NaPi-IIB or NaPi-2b in rat/mouse, and NaPi-3b in human, is closely related isoform of the NaPi-2 family.
It is expressed in small intestine and lung.
Type III NaPi transporters, originally described as a family of cell surface receptors for gibbon ape leukemia virus (GALV) and murine amphotropic retrovirus (A-MuLv), share very low (<20%) sequence homology with Type I and II proteins, and are found in most tissues.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:5000 using ECL.
ELISA: 1:10,000-50,000.
Coat 10-100ng of N0035-26B control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes mouse NaPi-2B. No significant sequence homology is observed with NaPi-2a or other Pi-transporters (Type-I, III, or IV) transporters. Recommend using human N0035-26 NaPi-2B for human studies.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 15aa within the cytoplasmic, C-terminus of mouse NaPi-2B, conjugated to KLH. Species Sequence Homology: rat; 93%.
