Ca2+ plays a critical role in intracellular signaling.
Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane.
NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane.
In most cells, 3 Na+ are exchanged for 1 Ca2+.
In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2).
NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus.
The N-terminal signal peptide is cleaved off from the mature exchanger protein.
NCX1 (rat 971aa, human 970aa, mouse 970aa) is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling, but is also present in most other tissues.
Alternative spicing of NCX1/NACA1 produces numerous tissue specific isoforms (heart NACA1; Kidney NACA2, -3, and -7; brain NACA4-6).
NCX2 (rat 921aa) is restricted to brain and skeletal muscle.
NCX3 (rat 927aa) shares 73-75% with NCX1 and NCX2.
NCX3 is also restricted to brain and muscle
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml (ECL)ELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes rat NCX. Does not have significant sequence homology with other NCX.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to a 20aa sequence within the N-terminus, extracellular domain 1 of rat NCX3, conjugated to KLH. Species Sequence Homology: mouse; 90%, human; 80%.
