84-1046-68　NCX2 (Sodium Calcium Exchanger) 100ul　N0569-02

特徴

• Ca2+ plays a critical role in intracellular signaling.
• Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane.
• NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane.
• In most cells, 3 Na+ are exchanged for 1 Ca2+.
• In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2).
• NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus.
• The N-terminal signal peptide is cleaved off from the mature exchanger protein.
• NCX1 (rat 971 aa, human 970 aa, mouse 970 aa) is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling, but is also present in most other tissues.
• Alternative spicing of NCX1/NACA1 produces numerous tissue specific isoforms (heart NACA1; Kidney NACA2, -3, and -7; brain NACA4-6).
• NCX2 (rat 921 aa) is restricted to brain and skeletal muscle.
• NCX3 (rat 927 aa) shares 73-75% with NCX1 and NCX2.
• NCX3 is also restricted to brain and muscle.
• Applications:Suitable for use in ELISA andWestern Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:5000ELISA: 1:10,000-1:50,000.
• Control peptide (Cat #N0569-25) can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.