Ca2+ plays a critical role in intracellular signaling.
Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane.
NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane.
In most cells, 3 Na+ are exchanged for 1 Ca2+.
In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2).
NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus.
The N-terminal signal peptide is cleaved off from the mature exchanger protein
NCX1 (rat 971 aa, human 970aa, mouse 970aa) is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling, but is also present in most other tissues.
Alternative spicing of NCX1/NACA1 produces numerous tissue specific isoforms (heart NACA1; Kidney NACA2, -3, and -7; brain NACA4-6).
NCX2 (rat 921aa) is restricted to brain and skeletal muscle.
NCX3 (rat 927aa) shares 73-75% with NCX1 and NCX2.
NCX3 is also restricted to brain and muscle.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 0.5-1ug/ml.
Control peptide N0560-20B can be used to coat ELISA plates at 1ug/ml
Immunohistochemistry (PFFE): 5-20ug/ml.
4% paraformaldehyde
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified over the peptide-Sepharose column
Form：Supplied as a lyophilized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes rat NCX1 (Sodium Calcium Exchanger). No significant sequence homology is detected with other NCX.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide consisting of 18aa sequence within the N-terminus of mature rat NCX1 conjugated to KLH. Species sequence homology: mouse-100%, feline-77%, human, rabbit, monkey, bovine-72%, chicken-66%.
