Ca2+ plays a critical role in intracellular signaling.
Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane.
NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane.
In most cells, 3 Na+ are exchanged for 1 Ca2+.
In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2).
NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus.
The N-terminal signal peptide is cleaved off from the mature exchanger protein.
NCX1 (rat 971 aa, human 970 aa, mouse 970 aa) is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling, but is also present in most other tissues.
Alternative spicing of NCX1/NACA1 produces numerous tissue specific isoforms (heart NACA1; Kidney NACA2, -3, and -7; brain NACA4-6).
NCX2 (rat 921 aa) is restricted to brain and skeletal muscle.
NCX3 (rat 927 aa) shares 73-75% with NCX1 and NCX2.
NCX3 is also restricted to brain and muscle.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The 18 AA rat control peptide 100% conserved in mouse, rat, 77% in feline, 72% in human, rabbit, monkey, bovine and 66% in chicken NCX1. No significant sequence homology is detected with other NCX. The control peptide is available to confirm specificity of antibodies
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：An 18 AA Peptide sequence near the N-terminus of mature rat NCX1 (1). The peptide was coupled to KLH
