Ca2+ plays a critical role in intracellular signaling.
Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane.
NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane.
In most cells, 3 Na+ are exchanged for 1 Ca2+.
In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2).
NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus.
The N-terminal signal peptide is cleaved off from the mature exchanger protein.
In vertebrate photoreceptors, some neurons, and certain other cells, 3 K+ are transported in the same direction as Ca2+, with coupling ratio of 4 Na+ to 1 Ca2+ plus 1 K+ by new class of exchangers called NCKX for K+-dependent Na/Ca+2 exchangers.
NCKX share the general topology and functional properties of NCX proteins.
Rod exchangers also contains a large hydrophilic segment at the its N-terminus that is not found in NCX.
NCKX1 (human 1099/1081 aa, bovine 1199 aa) is expressed in retinal rod cells.
NCKX2 (rat 670 aa) shares 80% identity with NCKX1.
The two hydrophilic loops are also much shorter in NKKX2 than in NCKX1, accounting largely for the difference between the size of the two proteins.
NCKX2 is expressed in various regions of the brain (striatum, parietal cortex, cerebellum, hippocampus, etc).
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The 18 AA human control peptide is 57% conserved in bovine NCKX1. No significant sequence homology is detected with rat NCKX1 other NCX or NCKX2. The control peptide is available to confirm specificity of antibodies
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 21 AA Peptide sequence near the N-terminus of mature human NCKX1 (1). The peptide was coupled to KLH
