84-1046-56　［取扱停止］NBC3 (HCO3 Cotransporters 3) 100ug　N0538-08

特徴

• Bicarbonate, along with CO2, is the major pH buffer of biological fluids.
• The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases.
• Several organs specialize in the mobilization of HCO3-.
• The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions.
• The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma.
• To prevent a massive acidosis, the proximal tubules (PT) cells in the nephron subsequently reabsorb virtually all of HCO3-, as well as most of the filtered Na+, Cl-, and H2O.
• A great majority of HCO3- reabsorption occurs via trans-cellular coupling of the luminal Na-H+ exchanger 3 and Na+-H+ ATPase with the basolateral Na+ HCO3- cotransporters (NBC).
• NBC3 (SLC4A); mouse (1089 aa, human 1044 aa) has 3 three transcripts, which are expressed in a tissue specific manner (9 kb in brain, placenta, lung, liver, muscle, kidney, pancreas, stomach, thyroid, and adrenal etc).
• NBC3 has two variants.
• NBC3 is 56% identical with NBC1 and 76% with NBC2.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml (ECL)
• ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide:N0538-10: NBC3, Human (HCO3 Cotransporters 3)
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.