84-1046-55　［取扱停止］NBC3 (HCO3 Cotransporters 3) 100ul　N0538-06

特徴

• Bicarbonate, along with CO2, is the major pH buffer of biological fluids.
• The inter-conversion of CO2 to HCO3- is mediated by family of carbonic anhydrases.
• Several organs specialize in the mobilization of HCO3-.
• The pancreatic ducts move CO2 and HCO3- from the blood into pancreatic secretions.
• The kidneys reabsorb massive amount of HCO3- daily (amount of NaHCO3 contained in 1 Ib of baking soda) through the glomerular flirtation of ~180 liters of plasma.
• To prevent a massive acidosis, thebe tissue specific.
• Physiologically, NBC is electrogenic, Na+ and HCO3- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS).
• The NBC family of proteins are 30-35% related to anion exchangers (AE2 and AE3; SLC4A1-SLC4A3) and display the same protein topology: (a) At least 10 TM domains with both the N and C-termini predicted to be intracellular, (b) presence of a large, glycosylated, extracellular loop between TM5 and TM6; and (c) the lysine residues are conserved at predicted DIDS-reactive sites.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:100,000.
• Using 50-100ng of control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide:N0538-10: NBC3, Human (HCO3 Cotransporters 3)
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.