Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
A band of 95-110kD has been detected in brush border membrane (2)
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：chicken
Source Antibody：Opossum
Grade：Affinity Purified
Purity：Affinity purified over the control-peptide Sepharose
Form：Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Specificity：Peptide is specific for opossum Napi-4/npt2a. This sequence is not conserved in mouse, rat, human and other species Npt2a. No significant sequence homology is observed with other Pi-transporters (Type-I, III, or IV) transporters. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity
Isotype：IgY
Calc Applications Abbrev：E WB
Immunogen：A 20 AA synthetic peptide within the N-terminus region (71-86 aa) of Opossum NaPi-4/NPT2a was synthesized, conjugated to KLH
