Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1).
Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1).
Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues.
It is dramatically up-regulated by iron starvation in the intestine.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
ELISA: 1:10,000-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:N0020-02A Natural Resistance Associated Macrophage Protein, Rat, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid, PBS, 40% glycerol.
Specificity：Recognizes rat NRAMP2. Species cross-reactivity: Species sequence homology: mouse-100%, human-94%, zebra fish -73% This sequence is common in NRAMP2 with or without IRE isoforms. This antibody will not distinguish between these isoforms.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：A 19 AA Peptide sequence located at the predicted cytoplasmic loop # 4 of rat NRAMP2 (1) (KLH)
