Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1).
Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1).
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL techniqueELISA: 0.5-1ug/ml Use 1ug/ml control peptide/wellImmunohistochemistry: 5-20ug/ml.
Use paraformaldehyde fixed sections
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:N0020-01: Natural Resistance Associated Macrophage Protein, Rat (Nramp2)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes rat Natural Resistance Associated Macrophage Protein (Nramp2). This sequence is common in NRAMP2 with or without IRE isoforms. This antibody will not distinguish between these isoforms.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：A 19 aa synthetic peptide sequence located at the predicted cytoplasmic loop #4 of rat NRAMP2 was synthesized, coupled to KLH. Species sequence homology: 100% mouse, 94% human, 73% zebra fish NRAMP2.
