Escherichia coli MutY is an adenine DNA glycosylaseactive on DNA substrates containing A/G, A/8-oxoG, or A/Cmismatches and also has a weak guanine glycosylaseactivity on G/8-oxoG-containing DNA.
The N-terminaldomain of MutY, residues 1-226, has been shown to retaincatalytic activity.
The post-replicative adenine:8-oxo-7,8-dihydroguanine (GO) mismatch is crucial for G:C to T:Atransversion.
This mismatch is corrected by Escherichiacoli MutY which excises the adenine from A:GO.
Acandidate gene coding for the human counterpart of MutYhas been cloned as hMYH (535 aa) (1).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:10,000-50,000.
Using 50-100ng control peptide/well
Western Blot: 1:1000-1:5000.
Optimal dilutions to be determined by the researcher.
Control Peptide: M9555-01
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity：Recognizes human mutY at ~55kD. The human peptide sequence has no significant homology with E. coli mutY.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A 19-aa peptide near the C-terminus of human mutY (KLH).
