84-1046-25　mutY 100ul　M9557-02

特徴

• Escherichia coli MutY is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA.
• The N-terminal domain of MutY, residues 1-226, has been shown to retain catalytic activity.
• The post-replicative adenine:8-oxo-7,8- dihydroguanine (GO) mismatch is crucial for G:C to T:A transversion.
• This mismatch is corrected by Escherichia coli MutY which excises the adenine from A:GO.
• A candidate gene coding for the human counterpart of MutYhas been cloned as hMYH (535 aa) (1).
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 ELISA:1:10,000-50,000 .
• Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Control Peptide: M9555-01Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.