Escherichia coli MutY is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA.
The N-terminal domain of MutY, residues 1-226, has been shown to retain catalytic activity.
The post-replicative adenine:8-oxo-7,8-dihydroguanine (GO) mismatch is crucial for G:C to T:A transversion.
This mismatch is corrected by Escherichia coli MutY which excises the adenine from A:GO.
A candidate gene coding for the human counterpart of MutY has been cloned as hMYH (535 aa) (1).
Human MutY is approximately 55kD.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000
ELISA: 1:5000-20,000 using 50-100ng control peptide (M9559-01)/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes MutY. The human peptide sequence has no significant homology with E. coli mutY (1).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：An 18-aa peptide near the N-terminus of human mutY (1). Peptide was coupled to KLH.
