Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1).
Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1).
Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues.
It is dramatically up-regulated by iron starvation in the intestine.
Applications:Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml.
Coat with 1ug/ml Control Peptide
Western Blot: 1-10ug/ml (ECL)Immunofluorescence: 5-10ug/ml using confocal microscopy
Immunohistochemistry: 5-20ug/ml.
Use paraformaldehyde fixed tissues
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:M4695-50A: Metal Transporter Protein, Mouse, Control Peptide (MTP1)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes mouse Metal Transporter Protein. Does not distinguish between isoforms ferroprotein 1/IREG1, ferroprotein 1 and IRG1/ferroprotein 1.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide consisting of 19aa sequence located within the predicted cytoplasmic C-terminus of mouse MTP1 (KLH). Sequence Homology: Mouse ferroprotein 1/IREG1: 100%; rat ferroprotein 1: 90%; human and chimp ferroprotein 1: 78% and rabbit MTP1/IREG1/ferroprotein 1: 73%.
