84-1046-19　Motilin 100ul　M4677-72

特徴

• Transport of nutrients through the digestive system is highly dependent on gastrointestinal (GI) motility.
• GI motility disorders include gastroesophageal reflux disease, gastroparesis (diabetic and post surgical), irritable bowel syndrome, and constipation.
• Motilin, a 22-amino acid peptide hormone that is secreted by enterochromaffin cells of the small intestine, influences gastric motility by inducing interdigestive (phase III) antrum and duodenal contractions.
• Most recently, an orphan GPCR related to growth hormone secretagogues receptor (GHS-R) has been isolated and characterized from human stomach as the motilin receptor (MTLR or GPR38; 52% identity with GHS-R).
• The structurally unrelated macrolide antibiotic erythromycin is known to have Motilin-like side effects, including vomiting, nausea, diarrhea, and abdominal muscular discomfort.
• MTLR is expressed in enteric neurons of the human duodenum and colon.
• Interestingly, the macrolide antibiotic erythromycin also binds to MTLR, providing a molecular basis for its effects on the human GI tract.
• Motilin gene (human chromosome 6p21.2) consists of 5 exons spanning approximately 9 kb of genomic DNA.
• Exon 1 encodes the 5-prime untranslated portion of the motilin mRNA.
• Exons 2 and 3 encode the signal peptide and the 22-amino acid motilin peptide.
• The 22-aa mature motilin peptide is highly conserved across species.
• The motilin prohomrone consists of 115 amino acids and includes a 25-residue N-terminal signal peptide followed by the 22-amino-acid motilin sequence and a long, 68-residue Motilin-related C-terminal peptide.
• Proteolytic processing of promotilin to motilin occurs at a very unusual and rare "Lys-Lys" sequence.
• High-affinity binding sites for motilin have been detected in the GI tract of humans and other species and in the central nervous system of rabbits.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilutions:ELISA: ~1:100,000 using 50-100ng control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide:M4677-70: Motilin, Human (Control Peptide)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.