Transport of nutrients through the digestive system is highly dependent on gastrointestinal (GI) motility.
GI motility disorders include gastroesophageal reflux disease, gastroparesis (diabetic and post surgical), irritable bowel syndrome and constipation.
Motilin, a 22aa peptide hormone that is secreted by enterochromaffin cells of the small intestine, influences gastric motility by inducing interdigestive (phase III) antrum and duodenal contractions.
Most recently, an orphan GPCR related to growth hormone secretagogues receptor (GHS-R) has been isolated and characterized from human stomach as the motilin receptor (MTLR or GPR38; 52% identity with GHS-R).
The structurally unrelated macrolide antibiotic erythromycin is known to have Motilin-like side effects, including vomiting, nausea, diarrhea and abdominal muscular discomfort.
MTLR is expressed in enteric neurons of the human duodenum and colon.
Interestingly, the macrolide antibiotic erythromycin also binds to MTLR, providing a molecular basis for its effects on the human GI tract.
MTLR/GPR38 (human chromosome 13q14-q21) encodes a protein with all the features of GPCR, including seven predicted TM domains.
MTLR is alternatively spliced.
MTLR1A/GPR38-A is 412aa and GPR38-B is 386aa with 5 TM domains.
MTLR is 52% identical with the recently cloned and characterized GHS-R or Ghrelin receptor.
MTLR gene is conserved distantly in evolution.
GPR38 is ~54% identical with a related gene (75E7) in the teleost puffer fish Spheroides nephelus which evolved ~400 million years ago.
Expression GPR38 was found in human enteric neurons of the human duodenum and colon, thyroid and bone marrow.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
ELISA: ~1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:M4677-50: Motilin Receptor, Human (MTLR, GPR38)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid, neat serum, 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes human MTLR/GPR38. Due to alternative splicing, GPR38B form does not contain the MTLR sequence. No significant homology with GHS-R. MTLRs sequences from other species are not yet available.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide 19aa sequence from the cytoplasmic, C-terminus of human MTLR (KLH).
