Transport of nutrients through the digestive system is highly dependent on gastrointestinal (GI) motility.
GI motility disorders include gastroesophageal reflux disease, gastroparesis (diabetic and post surgical), irritable bowel syndrome and constipation.
Motilin, a 22aa peptide hormone that is secreted by enterochromaffin cells of the small intestine, influences gastric motility by inducing interdigestive (phase III) antrum and duodenal contractions.
Most recently, an orphan GPCR related to growth hormone secretagogues receptor (GHS-R) has been isolated and characterized from human stomach as the motilin receptor (MTLR or GPR38; 52% identity with GHS-R).
The structurally unrelated macrolide antibiotic erythromycin is known to have Motilin-like side effects, including vomiting, nausea, diarrhea and abdominal muscular discomfort.
MTLR is expressed in enteric neurons of the human duodenum and colon.
Interestingly, the macrolide antibiotic erythromycin also binds to MTLR, providing a molecular basis for its effects on the human GI tract.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
ELISA: ~1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:M4677-50: Motilin Receptor, Human Control Peptide (MTLR, GPR38)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity：Recognizes human MTLR/GPR38. Due to alternative splicing, GPR38B form does not contain the MTLR sequence. No significant homology with GHS-R.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide 19aa sequence from the cytoplasmic, C-terminus of human MTLR (KLH).
