Circadian rhythm is one of the most fascinating and complex biological phenomenon's.
The circadian clock controls biological activities on daily light-dark cycles in species from cyanobacteria to humans.
The circadian clock has three major components: A photoactive pigment (chromophore) for sensing light and transmitting light signals, the circadian clock that oscillates every ~24-hrs, and the genes controlled by the circadian clock to bring about the physiological and behavioral changes.
Several genes (Drosophila Clock Per, Tim; mammalian Per1, Per2, Per3, MOP3 and MOP4, and BMAL1) have been linked to rhythmicity or circadian behavior of living organisms.
However, very little is known about the mammalian photosensory molecules.
Basic-helix-loop-helix-PAS orphan protein, MOP3 (Members Of PAS Superfamily; also known as BMAL1/JAP3/PAS3; 626 aa) is a general dimerization partner for several PAS superfamily of transcription regulators.
MOP3 interacts with MOP4 (also known as NPAS2; 626 aa), Clock, HIF1a, and HIF2a.
MOP4 is a brain specific homolog of Clock.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：chicken
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：Human sequence is 78% conserved in mouse and 73% in mouse MOP4/NPAS2. Antibody crossreactivity with MOP4 from various species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity
Isotype：IgY
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 19-aa peptide internal sequence of Human MOP4 (1) was synthesized coupled to KLH
