Circadian rhythm is one of the most fascinating and complex biological phenomenon's.
The circadian clock controls biological activities on daily light-dark cycles in species from cyanobacteria to humans.
The circadian clock has three major components: A photoactive pigment (chromophore) for sensing light and transmitting light signals, the circadian clock that oscillates every ~24-hrs, and the genes controlled by the circadian clock to bring about the physiological and behavioral changes.
Several genes (Drosophila Clock Per, Tim; mammalian Per1, Per2, Per3, MOP3 and MOP4, and BMAL1) have been linked to rhythmicity or circadian behavior of living organisms.
However, very little is known about the mammalian photosensory molecules.Basic-helix-loop-helix-PAS orphan protein, MOP3 (Members Of PAS Superfamily; also known as BMAL1/JAP3/PAS3; 626 aa) is a general dimerization partner for several PAS superfamily of transcription regulators.
MOP3 interacts with MOP4 (also known as NPAS2; 626 aa), Clock, HIF1a, and HIF2a.
MOP4 is a brain specific homolog of Clock.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: M4591-02B
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 0.1%BSA and 40% glycerol.
Specificity：Recognizes human MOP3. Sequence is 100% conserved in related proteins from other species (rat TIC; human bMAL1a & bMAL1b, PAS3, JAP3 and mouse Arnt3).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo Rt
Immunogen：A 15-aa peptide sequence within the C-terminus of Human MOP3 was synthesized coupled to KLH
