Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism.
Lactic acid is produced as the end product of glycolysis.
Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions.
All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia.
Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis.
Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis.
Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells.
Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs).
At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner
MCT1 (also known as MOT1 or SLC16A1 or MEV; mouse 493aa, rat 49 aa, human 500aa; ~95% identity) is a membrane protein containing 12 transmembrane proteins.
MCT1 is most closely related to MCT2 (~65% identity, whereas homolog with other MCT2-MCT8 isoforms is less (~35-53%).
MCT1 has very wide tissue distribution.
MCT1/MOT1 is ubiquitously expressed but is especially prominent in heart and red muscle.
It is upregulated in response to increased work, suggesting an important role in lactic acid oxidation.
It is the major isoform in tumor cell and erythrocytes.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:M4470-03E: Monocarboxylate Transporter 1, Human Control Peptide (MCT1)
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes human MCT1. No significant sequence homology was found with other MCTs
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 19aa near the C-Terminal, cytoplasmic domain of human MCT1, conjugated to KLH. Species Sequence Homology: hamster; 63%, mouse; 57%
