Many tumor promoting/causing agents (BCNU, CCNU, DTIC, procarbazine, temozolomide, etc) generate , lethal cross-links at the O6-alkylguanine position in DNA.
MGMT removes alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to its cysteine residue, thereby irreversibly inactivating MGMT.
Inactivated MGMT is rapidly degraded.
Therefore, MGMT levels serve as an indicator of DNA damage.
Tumors with high level of MGMT are likely to be drug resistant and low or no MGMT may make the tumor more responsive to chemotherapy.
In normal cells, abnormally low level of MGMT may make normal cells/tissue more susceptible to tumor promoting/alkylating agents
Applications:Suitable for use in ELISA, Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000.
Use 50-100ng antigen/well (M3884-92D).
Western Blot: 1:500-1:20,000
Optimal dilutions to be determined by researcher.
Positive Controls:Liver and many tumor cells (Molt-4, Raji, HeLa, HT-292)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human MGMT at ~25kD. Poor crossreactivity with mouse and rat MGMT proteins in Western Blot.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Purified recombinant human MGMT protein (~25kD, 207aa) expressed in E. coli.
