Melanocortins are regulatory peptides formed by post-translational processing of pro-opiomelanocortin.
Melanocortin peptides have been suggested to perform a variety of physiological roles ranging from control of behavior, memory, neurotrophic properties, antipyretic and modulation of immune system, etc.
Their binding sites have been found distributed in tissues ranging from lachrymal and submandibular glands, pancreas, adipose tissue, bladder, duodenum, spleen, brain, gonadal tissues and malignant melanoma tumors.
Five melanocortin receptors have been characterized to date.
These include melanocyte-stimulating hormone receptor (MSH or MC1-R), corticoaderenal-specific ACTH receptor (MC2-R), melacortin-3 (MC3-R), melanocortin-4 (MC4-R) and melanocortin-5 receptor (MC5-R).
MC3-R and MC4-R are distributed in brain whereas MC5-R has a broad distribution.
Mouse, rat , and human Melanocortin receptor 5 (MC5-R) are 325 amino acid transmembrane protein belonging to the family of G-Protein coupled receptors.
It is a receptor for MSH (a,b,g) and ACTH.
It is found in brain but not in melanocyte.
MCR-5 may be involved in immunomodulation properties of melanocortins.
mediate t, placenta, and gut tissues.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:1000,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: M2868-45
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes Melanocortin Receptor 5. No significant sequence homology with other receptors. Species sequence homology: Human and rat: 93% (14/15 aa); Porcine, sheep and bovine: 73% (11/15 aa); chicken: 63%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Rt
Immunogen：A 15-aa synthetic peptide near the N-terminus of Human MC5-R, coupled to KLH.
