Melanocortins are regulatory peptides formed by post-translational processing of pro-opiomelanocortin.
Melanocortin peptides have been suggested to perform a variety of physiological roles ranging from control of behavior, memory, neurotrophic properties, antipyretic and modulation of immune system, etc.
Their binding sites have been found distributed in tissues ranging from lachrymal and submandibular glands, pancreas, adipose tissue, bladder, duodenum, spleen, brain, gonadal tissues and malignant melanoma tumors.
Five melanocortin receptors have been characterized to date.
These include melanocyte-stimulating hormone receptor (MSH or MC1-R), corticoaderenal-specific ACTH receptor (MC2-R), melacortin-3 (MC3-R), melanocortin-4 (MC4-R) and melanocortin-5 receptor (MC5-R).
MC3-R and MC4-R are distributed in brain whereas MC5-R has a broad distribution.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using chemilluminescenceELISA: 1:10,000-1:100,000Immunohistochemistry and Immunoflourescence: 2-20ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Immunogen：Synthetic peptide corresponding to 18aa from the C-terminal, cytoplasmic domain of mouse MC3-R, conjugated with KLH. Species Sequence Homology: 83% (15/18 aa) with rat and human and 77% sheep
