Melanocortins are regulatory peptides formed by post-translational processing of pro-opiomelanocortin.
Melanocortin peptides have been suggested to perform a variety of physiological roles ranging from control of behavior, memory, neurotrophic properties, antipyretic and modulation of immune system, etc.
Their binding sites have been found distributed in tissues ranging from lachrymal and submandibular glands, pancreas, adipose tissue, bladder, duodenum, spleen, brain, gonadal tissues and malignant melanoma tumors.
Five melanocortin receptors have been characterized to date.
These include melanocyte-stimulating hormone receptor (MSH or MC1-R), corticoaderenal-specific ACTH receptor (MC2-R), melacortin-3 (MC3-R), melanocortin-4 (MC4-R) and melanocortin-5 receptor (MC5-R).
MC3-R and MC4-R are distributed in brain whereas MC5-R has a broad distribution.
Melanocyte Stimulating Hormone Receptor (MSH-R), also called Melanotropin Receptor, or Melanocortin-1 Receptor (MC1-R), is a 315 amino acid transmembrane protein belonging to the family 1 of G-Protein coupled receptors.
It is a receptor for MSH (a,b,g) and ACTH.
Its activity is mediated by G-proteins which activate adenylate cyclase.
It is found in Melanocytes and corticoaderenal tissue as well as various tissues like adrenal gland, leukocytes, lung, lymph node, ovary, testis, pituitary, placenta, spleen and uterus.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 ELISA: 1:10,000-1:100,000Optimal dilutions to be determined by the researcher.
Control Peptide:M2868-05 Melanocortin Receptor 1, Control Peptide Mouse
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes mouse Melanocortin Receptor 1. Species sequence homology: human-70%, equine, bovine and sheep-66%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：21aa from the C-terminal, cytoplasmic domain of the murine MC1-R ( KLH).
