The cyclic neuropeptide, Melanin-Concentrating Hormone (MCH), plays an important role in food intake and energy balance.
MCH stimulates feeding.
it is up regulated in obese mice and during fasting.
Animals lacking MCH eat less and are leaner.
A receptor for MCH (MCHR) has been cloned.
Human MCH receptor is 402aa membrane protein described as an orphan G-protein coupled receptor SLC-1 or GPR24, somatostatin receptor-like protein.
It has 7 transmembrane domains, a Dry motif at the boundary of TM3 and the 2nd intracellular loop, a consensus site for N-linked glycosylation at the N-terminus.
MCHR is expressed in ventromedial and dorsomedial nuclei of the hypothalamus.
An orphan G-protein coupled receptor (SLC-1, GPR24) has been identified as the receptor of MCH.
MCH receptor (MCH1R; human 402aa, rat 353aa) is predicted to contain 7 transmembrane domains.
Moderate levels of MCHR are also found in eye and skeletal muscle, tongue and pituitary.
MCHR binds MCH with sub-nanomolar affinity.
It is stimulated by MCH to mobilize intracellular Ca2+ and reduce forskolin-elevated cAMP levels.
A second human MCH receptor (MCH2R) has been cloned and characterized.
MCH2R gene encodes a 340aa protein with 38% identity with MCH1R.
It has the basic characteristics of GPCR and belongs to class 1 GPCR superfamily.
MCH2R transfected cells responds to MCH with an increase in intracellular Ca2+ levels and increased cellular extrusion of protons.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:M2867-75C: Melanin Concentrating Hormone Receptor1, Human, Control Peptide (MCHR1)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human MCHR1. No significant sequence homology is seen with MCHR2 or with other GPCRs. Species Sequence Homology: Rat, mouse, monkey MCHR1: 100%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (16aa) near the cytoplasmic C-terminus of human MCHR/GPR24/SLC-1 (KLH).
