Melanin-concentrating hormone (MCH) is a cyclic neuropeptide (human/rat 19aa) that regulates a variety of functions in mammalian brain, in particular stimulation of feeding behavior.
MCH is synthesized mainly in the lateral hypothalamus and zona incerta.
Its level is unregulated in the hypothalamus of genetically obese mice.
Mice lacking MCH eat less and are leaner.
MCH is thought to influence feeding and energy balance by acting downstream of leptin and the melanocortin system.
Molecular mechanisms of these diverse actions of MCH remain poorly understood
An orphan G-protein coupled receptor (SLC-1, GPR24) has been identified as the receptor of MCH.
MCH receptor (MCHR; human 402aa, rat 353aa) is predicted to contain 7 transmembrane domains, a feature typical of G-protein coupled receptors.
It is primarily expressed in the ventromedial and dorsomedial nuclei of the hypothalamus.
Moderate levels of MCHR are also found in the eye and skeletal muscle, tongue and pituitary.
MCHR binds MCH with sub-nanomolar affinity.
It is stimulated by MCH to mobilize intracellular Ca2+ and reduce forskolin-elevated cAMP levels.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:M2867-70B
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human Melanin Concentration Hormone (MCH). No significant sequence homology is seen with other peptide hormones. Species sequence homology: Mouse, rat: 100%; fish: 76%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic, full length human MCH was purified by HPLC, cysteine oxidized (Cys 7, Cys16), AND KLH coupled (DFD MLR CML GRV YRP CWQ V).
