MDA (Malondialdehyde) is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism.
MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA.
MDA-modified proteins may show altered physico-chemical behavior and antigenicity.
Antibodies to MDA will help to visualize the MDA-adducts.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000ELISA: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：goat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from 0.05% sodium azide. Reconstitute with 100ul sterile dH2O.
Specificity：Recognizes Malondialdehyde (MDA)-protein adducts in all species
