84-1045-72　Mahogany (Attractin) 50ul　M2108-01

特徴

• Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity.
• In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub).
• More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned.
• The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti.
• Agouti protein (132aa in human) is normally expressed in skin but its ubiquitous expression, such as in rare allele lethal yellow, causes yellow coat., linear growth, immune defects and obesity.
• Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1 and possibly MC2R and MC4R).
• The Mahogany (mg) mutations suppress the effect of lethal yellow including obesity.
• The Mahogany locus does not suppress the obese phenotype of the MC4R null allele or those of monogenic obese models (Lepdb, tub, and Cpefat).
• However, mahogany can suppress diet-induced obesity.
• Mahogany (mg) gene, Mgca, encodes a 1428 aa single transmembrane protein that is expressed in many tissues including pigment cells and hypothalamus.
• The extracellular domain of the Mgca protein is the orthologue of human attractin, a circulating molecule produced by activated T-cells implicated in immune cell interaction.
• The short cytoplasmic tail contains a site that is conserved between C.
• elegans and mammals.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-100,000; using 50-100ng of control peptide (Cat # M2108-03)/well.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.