Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1).
Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568aa, ~65% identity with NRAMP1).
Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues.
It is dramatically up-regulated by iron starvation in the intestine.
NRAMP2 gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs).
Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR.
The IRE is an RNA secondary structure present in the 5'- or the 3'-UTR of animal mRNAs encoding proteins involved in iron metabolism.
The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) are replaced by a novel 25aa segment and codes for a distinct 3' UTR lacking the IRE.
The two isoforms are differentially localized and regulated in GI tract and kidney.
It has recently been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgarde (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml.
Coat ELISA plates at 1ug/ml of Control Peptide
Optimal dilutions to be determined by the researcher.
Control Peptide:N0020-02A: Natural Resistance Associated Macrophage Protein, Rat, Control Peptide (Nramp2)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes rat NRAMP2 with IRE
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to 17aa located near the C-terminal, cytoplasmic domain of rat NRAMP2 conjugated with KLH. This sequence is 100% homologous with mouse Nramp2 with IRE and is absent in NRAMP2 without IRE. No significant sequence homology exists with NRAMP1.
