84-1045-69 Livin (ML-IAP) 100ul L3180-21

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特徴

  • Apoptosis or programmed cell death is a fundamental cellular process that is essential for normal tissue development and abnormal growth such as cancer, neurodegeneration, autoimmune diseases, and angiogenesis, etc.
  • Apoptosis is driven by specialized proteases known as caspases (Cysteine Aspartase).
  • The inhibitor-of-apoptosis protein (IAPs) by virtue of inhibiting caspase activity has widespread anti-apoptotic potential family.
  • IAPs are characterized by one or more repeats of a highly conserved ~70 amino acid domain termed the baculoviral IAP repeat (BIR).
  • There are at least five human IAP family members, c-IAP1, c-IAP2, XIAP, NAIP, and survivin.
  • All of the human IAP family members, with the exception of NAIP, have been shown to interact with specific cysteine proteases, or caspases.
  • The caspases are synthesized as inactive zymogen forms which upon apoptotic stimulation are proteolytically processed in a sequential manner into their active heterotetrameric forms.
  • The BIR domain is critical motif for anti-apoptotic activity and interaction with caspases.
  • Many of the IAPs also contain a RING domain near their COOH-termini that is not required for the anti-apoptotic activity.
  • A novel member of the IAPs, termed livin (also known as IAP kidney or KIAP; IAP melanoma or MLIAP), encodes a protein with a single BIR domain and a COOH-terminal RING domain.
  • Expression of livin inhibited apoptosis by a number of stimuli.
  • Livin can bind to caspases and it could inhibit the proteolytic processing of caspase-9 in vitro.
  • Livin gene (chromosome 20q13.3) encodes a 280 amino acid protein.
  • It has 25-35% identity with other IAP family members (c-IAP1, c-IAP2, XIAP, NAIP, and surviving).
  • At a structural level it was similar to survivin with respect to having just a single BIR domain.
  • However, livin did not have a coiled-coil domain like survivin, but rather contained a COOH-terminal RING domain found in c-IAP1, c-IAP2, and XIAP.
  • Livin gene has two splicing variants that contain open reading frames of 298 and 280 amino acids and both contained a single copy of baculovirus IAP repeat (BIR) and RING domain.
  • Both of the isoforms inhibit TNFa induced apoptosis in Jurkat cells.
  • Livin expression is low in adult tissues.
  • It is relatively expressed at a higher level in developmental tissues and in many cancer cells.
  • Livin was not detectable in most normal adult tissues with the exception of the placenta, but was present in fetal brain and in several cancer cell lines (melanoma-derived cell lines, G361 and SK-Mel29.
  • Human Livin (1-280 aa.
  • ~32kD) was expressed as his-tag protein in E.
  • colli and purified >95%.
  • For western blot +ve control, it is supplied in SDS-PAGE sample buffer (reduced).
  • Load ~10 ul/lane to visualize with antibodies.
  • Applications:Suitable for use in ELISA, Western Blotting.
  • Other applications not tested.
  • Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
  • Livin is ~32kD protein (1)
  • ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure.
  • Control peptide can be used to coat ELISA plates at 1ug/ml.
  • Optimal dilutions to be determined by the researcher.
  • Storage and Stability:May be stored at 4°C for short-term only.
  • For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
  • Freeze at -20°C.
  • Aliquots are stable for at least 12 months at -20°C.
  • For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
  • Further dilutions can be made in assay buffer.

仕様

  • Size:100ul
  • Host:rabbit
  • Source Antibody:human
  • Grade:Serum
  • Purity:Serum
  • Form:Supplied as a liquid, neat serum, 40% glycerol.
  • Specificity:Human control peptide has no significant sequence homology with other IAPs. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity.
  • Isotype:IgG
  • Calc Applications Abbrev:E WB
  • Calc Crossreactivity:Hu
  • Immunogen:15-aa peptide from human Livin (1); Designation
  • Shelf Life:1year
  • EU Commodity Code:30021010
  • この商品は法規制を確認しておりません。(法規制によって販売できない場合もございます)
  • 製品の仕様は予告なく変更になる場合がございます。最新仕様はメーカーホームページをご確認ください。
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アズワン品番 84-1045-69
型番 L3180-21
入り数 1個
標準価格 181,000円(税抜)
WEB価格
出荷予定日
アズワン在庫 [?]
サプライヤ在庫 [?]
数量

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