Mammalian kidney and liver are critical in maintaining physiological ionic environment.
Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion".
Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta.
Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues.
OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus)
Most recently, a novel liver-specific OAT, LST-1, has been cloned from human liver.
It has also been detected in mouse and rat liver.
No significant expression of LST-1 was detected in other tissues.
Human LST-1 (691 aa, 12 TM) shares 42% homology with human OATP, rat oatp1-3, and OATK-1, and 35% with PGT.
Phylogenetic analyses places LST-1 between oatp and PGT.
LST-1 has wide substrate specificity (taurocholate, conjugated steroids, PGE2, Thromboxane B2, leukotrienes, and T3).
Therefore, LST-1 may be more important for clearance of bile acids and organic anions in liver.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:5000ELISA: 1:10,000-50,000, Control peptide (cat # L3176-05) can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes human LST-1. No significant sequence homology is detected with other oatp1-3, OATP or PGT. Epitope: ~C-terminus
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 17aa from human Liver Specific Transporter (LST-1) conjugated to KLH.
