The actions of insulin culminate in changes in the phosphorylation state of a number of downstream targets including phosphatidylinositol 3-OH kinase (PI3-kinase).
Activation of PI3-kinase is central to insulin-stimulated phosphorylation in fat cells.
In adipocytes, PI3 is involved in activation of protein kinase B (PKB), glycogen synthase, transitional regulators, 4E-BP1, p70s6k and mTOR protein (mammalian target of rapamycin).
The kinase activity of mTOR functions in nutrient sensing pathway that maintains a proper balance of amino acid availability, protein synthesis and cell growth.
Most importantly, mTOR controls the phosphorylation of a newly discovered protein Lipin-1, required for normal adipose tissue development and metabolism.
The mutation in Lipin-1 gene results in immature adipocytes and thus in fatty liver dystrophy (fld) phenotype in mice and in lipodystrophy, a group of rare human diseases.
These phenotypes are characterized by a triglyceride-filled fatty liver, loss of body fat, hypertrigyceridemia, insulin resistance, increased susceptibility to atherosclerosis, reduced fertility, reduced plasma Leptin and a progressive neuropathy affecting peripheral nerves in adulthood.
Lipin defines a family of nuclear proteins containing at least three members in human and mouse: Lipin-1, Lipin-2 and Lipin-3.
All Lipin members contain a nuclear signal sequence, a highly conserved amino- (NLIP) and a carboxy-terminal (CLIP), domains
The human and mouse LPIN-2 genes have been mapped at chromosomes 18p and 17, respectively.
The Lipin 2 sequences from mouse and human are ~90% identical.
The overall amino acid sequence of mouse Lipin 2 (891aa) is ~48% identical to mouse Lipin 1(891aa) and Lipin 3 (848aa).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml; Control peptide (Cat.
# L2497-10B) can be used to coat ELISA plates at 1ug/ml
Western Blot: 1-10ug/ml using ECL
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes mouse Lipin 2. Reacts with mouse Lipin 2 under non-reducing conditions in Western Blot.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：14aa peptide of mouse Lipin 2, near the N-Terminal. No significant sequence homology is seen with Lipin 1, Lipin 3 or other proteins. Species Sequence Homology: Human - 69%.
