The actions of insulin culminate in changes in the phosphorylation state of a number of downstream targets including phosphatidylinositol 3-OH kinase (PI3-kinase).
Activation of PI3-kinase is central to insulin-stimulated phosphorylation in fat cells.
In adipocytes, PI3 is involved in activation of protein kinase B (PKB), glycogen synthase, transitional regulators, 4E-BP1, p70s6k and mTOR protein (mammalian target of rapamycin).
Kinase activity of mTOR functions in nutrient sensing pathway that maintains a proper balance of aa availability, protein synthesis and cell growth.
Most importantly, mTOR controls the phosphorylation of a newly discovered protein Lipin-1, required for normal adipose tissue development and metabolism.
The mutation in Lipin-1 gene results in immature adipocytes and thus in fatty liver dystrophy (fld) phenotype in mice and in lipodystrophy, a group of rare human diseases.
Besides Lipin-1, there are at least 2 more nuclear proteins (Lipin-2 and Lipin-3) in human and mouse lipin families All lipin members contain a nuclear signal sequence, a highly conserved amino-(NLP) domain and a carboxy-terminal (CLIP) domain.
The human and mouse LPIN-1 genes have been mapped at chromosomes 2p21 and 12, respectively.
A region (POMC) on this same chromosome also encodes pro-opiomelanocortin, a locus linked to total adiposity and the levels of plasma protein Leptin.
The mouse Lipin-1 exists as isoform 1(891aa, missing 241-273 aa) and isoform 2 or isoform b (924 aa).
It is a 140kD protein with many potential phosphorylated sites.
The overall aa sequence of mouse Lipin-1 is over 46 and 49% identical to mouse Lipin-2 and Lipin-3, respectively.
The seqs of human and mouse Lipin-1 are over 88% identical.
Lipin-1 mRNA is prominently expressed in adipose tissue, skeletal muscle and testis with lower expression in kidney, lung, brain and liver.
Applications: Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
Will recognize ~140kD mouse Lipin-1 under non-reducing conditions.
It is not clear if isoform 2 is also recognized with this antibody
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher
Control Peptide:L2497-01B Lipin 1, Mouse Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH7.5, 0.1% BSA, 40% glycerol.
Specificity：Recognizes mouse Lipin 1. Species Sequence Homology: rat (93%), human and chimpanzee (81%), chicken (75%). Epitope is around 120aa region that is well before the truncation site for isoform 1; therefore epitope is present in both isoforms 1 and 2. No significant sequence homology seen with Lipin-2, Lipin-3 or other proteins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic 16aa peptide sequence (aa111-126) at the N-terminus of mouse Lipin 1 (KLH coupled).
