84-1045-63　Leptin Receptor Gene Related Protein (OBGRP) 100ul　L1671-24C

特徴

• Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems.
• At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity.
• Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (167 amino acid, ~16kD) that controls body weight homeostasis.
• Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LR) that is expressed in several tissues including hypothalamus.
• The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain.
• The OBRa represents the initially identified mouse Ob-R (short form, 894 AA).
• OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165 AA).
• OBRb has ~300 aa intracellular tail.
• Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues.
• OBRe lacks the transmembrane domain.
• The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes.
• Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling.
• More recently, another variant of OBR, termed OB-RGRP (obese receptor gene related protein), has been identified that is distinct from the leptin receptor itself.
• OB-RGRP transcript contains the first two OBR gene 5'-untranslated exons, but then is alternatively spliced to two either leptin receptor or 131 aa protein OB-RGRP.
• The OB-RGRP is highly conserved in mouse, rat, human, yeast and C.
• elegans.
• OPB-RGRP is highly expressed in the hypothalamic regions that express the OBR mRNA.
• The OBR/B219, but not the OB-RGRP, is up-regulated in hypothalamus of ob/ob mice.
• OB-RGRP mRNA is also detected in heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• A new full-length cDNA encoding a new 131aa protein, leptin receptor overlapping transcript-like 1 gene (LEPROTL1), that is highly homologous with OB-RGRP (~70% identity with OB-RGRP).
• LEPROTL1 is also widely expressed and has a similar JAK binding site (Pro46-Ile-Pro48) that is conserved in homologous proteins.
• LEPROTL1 protein is predicted to display 3 TM domains with an extracellualr N-terminus and intracellular C-terminus.
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western: 1:1000 dilution of antiserum or 1-10ug/ml of affinity purified IgG.
• Optimal dilutions must be optimized
• ELISA: 1:10,000-50,000 10-100ng of control peptide/well.
• Optimal dilutions to be determined by the researcher.
• Control peptide, because of its low MW (<3kD), is not suitable for Western.
• It should be used for ELISA or antibody blocking experiments (use 5-10ug control peptide per 1ug of affinity purified IgG or 1ul antiserum) to confirm antibody specificity.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.