Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems.
At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity.
Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (167 amino acid, ~16kD) that controls body weight homeostasis.
Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LR) that is expressed in several tissues including hypothalamus.
The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain.
The OBRa represents the initially identified mouse Ob-R (short form, 894aa).
OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165aa).
OBRb has ~300 aa intracellular tail.
Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues.
OBRe lacks the transmembrane domain.
The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes.
Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml ELISA: 1:10,000-50,000; 10-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:L1671-24B: Leptin Receptor Gene Related Protein, Human Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes human OB-RGRP. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 15aa within the first putative intracellular domain of human OB-RGRP, conjugated to KLH. Species Sequence Homology: human LEPROTL1, 53%
