Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems.
At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity.
Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (146 amino acid, ~16kD) that controls body weight homeostasis.
Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LEPR) that is expressed in several tissues including hypothalamus.
The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain.
The OBRa represents the initially identified mouse Ob-R (short form, 894aa).
OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165aa).
OBRb has ~300 aa intracellular tail.
Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues.
OBRe lacks the transmembrane domain.
The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes.
Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling.
Leptin is structurally related to cytokine family and it activates cytokine-like signal transduction by stimulating the JAK-STAT pathway via the OBRb.
The absence of functional leptin in ob/ob and the long form OBRb in db/db mice due to abnormal splicing produces severe obesity.
The nature of leptin resistance in other obesity models is not clear.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-50,000 10-100ng of Control peptide (Cat # L1671-09B )/well
Western Blot: 1-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity：Recognizes mouse leptin receptor. Mouse sequence is common in all six alternatively spliced mouse/rat obese receptors (a-e forms). Has no significant homology to gp130.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 15aa near the N-terminus, extracellular domain of mouse OBR. Species Sequence Homology: human, porcine, monkey and African elephant OBR isoforms, 86%
